Figure 1.

CAFs promote the migration capacity of pancreatic cancer cells by promoting EMT. A. The effect of CM from CAFs on pancreatic cancer cell migration was detected using Transwell chambers in Capan1 and Panc1 cells. B. Morphological changes in Panc1 and Capan1 cells after culture with pancreatic fibroblasts. C. QRT-PCR analysis of E-cadherin and Vimentin levels in Panc1 and Capan1 cells treated as described in A. D. E-cadherin and Vimentin levels determined by western blotting in pancreatic cancer cells culture with CAF-CM. *P < 0.05, **P < 0.01.