Figure 1.
Immunostaining of NETs-formation induced by C. albicans. The isolated human neutrophils were incubated with C. albicans SC5314 (B1-3) or 3683 (C1-3) or PBS (A1-3) at a ratio of 5:1 (cells: yeasts) for 120 min. Then the samples were fixed, and stained with antibodies against human neutrophil elastase (green) and DAPI to detect DNA (blue). At last, the samples were observed and photographed with confocal microscope. White arrows point to neutrophils, red arrows point to NETs.