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Proliferation effect of C3H mouse-antigen specific mCD4. A. CFSE labeled memory CD4+ T cells were cultured in 96-well plates 105/well with control, con A. (4 µg/ml), C3H spleen cells (105/well). B. Cells were harvested 0 h, 24 h, 48 h, 72 h, and 96 h for proliferation index measurement. Proliferation index at 0 hour was considered as 1. C. Supernatants were collected for the detection of IL-2 by ELISA at 96 h.
