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Effect of CD8+ regulatory T cells on antigen specific memory CD4+ T cells (E/T). A. Qa-1-restricted CD8+ T cells were cultured with memory CD4+ T cells at various E/T ratio=1, 2, 4. B. Proliferative effect was detected by Flow Cytometery at 96 hour. C. IL-2 in supernatant was measured by ELISA at 96h. D. The relationship between supernatants IL-2 level and proliferation index of Memory CD4+ T cells.
