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Lysis function of CD8+ Tregs on memory CD4+ T cells. A. CFSE labeled memory CD4+ T cells and CD8+ T cells were incubated at various E/T ratio, accompanied by inactivated C3H spleen cells. B. Lysis of memory CD4+ T cells by CD8+ T cells. C. IFN-γ from cultured supernatants was detected by ELISA. D. The relationship between supernatants IFN-γ level and lysis of memory CD4+ T cells. (*, P<0.05; **, P<0.01; ***P<0.001).
