Figure 4.

Immunofluorescence microscopy demonstrated that nicousamide could significantly restore the expression of nephrin and podocin in glomerulus from diabetic rats, and kept the podocyte integrity, compared to weak staining from DN model group. A. Nephrin and podocin staining with monoclonal antibodies and FITC-conjugated second antibody. B. Bars show quantification of nephrin and podocin stained area. %: percentage of total glomerular area. Data are means ± SD. #P<0.05 vs. Normal; *P<0.05, **P<0.01 vs. DN model.