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Western blot results of nicousamide on protein expression of TGF-smad signaling pathway and RAGE. Compared to DN model group, nicousamide treatment significantly decreased the protein level of TGFβ1, p-TGFβ RII and p-smad2, but no remarkable influence on total TGFβ RII, total smad2 and RAGE. The figure includes representative figures of western blot and quantification analysis of protein levels by Image J.
