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MiR-221 inhibits Runx2 expression in C2C12 cells. A. Putative miR-221-binding sequence of Runx2 RNA. Mutation (Mut) was generated on the Runx2 RNA sequence in the complementary site for the seed region of miR-221. B. The expressions of RUNX2 in C2C12 cells transfected with miR-221 mimic or inhibitor detected by western blot analysis. *P < 0.05. C. The expressions of RUNX2 in C2C12 cells transfected with miR-221 mimic or inhibitor detected by RT-qPCR. *P < 0.05.
