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. 2017 Jan 15;9(1):126–135.

Figure 4.

Figure 4

RUNX2 is a direct target of the miR-221 in C2C12 cells. A. Overexpression or knockdown of miR-221 expression inhibited or enhanced the Renilla luciferase activities. C2C12 cells were cotransfected with miR-221 mimic/inhibitor or mimic/inhibitor control, and reporter plasmid containing the wild-type (WT) 3’-UTR of RUNX2. A luciferase activity assay was subsequently performed. *P < 0.05. B. C2C12 cells were cotransfected with the miR-221 mimic and reporter plasmid containing the WT or Mut 3’-UTR of RUNX2. A luciferase activity assay was subsequently performed. *P < 0.05.