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. 2017 Jan 15;9(1):146–154.

Figure 4.

Figure 4

CCND1 and CD44 are the target genes of miR-520a-3p in breast cancer cells. A. Binding sequences of CCND1 and CD44 with miR-520-3p. B. Luciferase activity of CCND1 3’UTR in Hs578T cells with miR-520a-3p mimics transfection. C. Luciferase activity of CD44 3’UTR in Hs578T cells with miR-520a-3p mimics transfection. D, E. miR-520-3p led to an obvious decrease in CCND1 and CD44 mRNA in Hs578T and BT20 cells using qRT-PCR. F. miR-520-3p led to an obvious decrease in CCND1 and CD44 protein levels in Hs578T and BT20 cells using Western blotting. G. miR-520-3p inhibitor led to an obvious increase in CCND1 and CD44 protein levels in Hs578T and BT20 cells using Western blotting. **P<0.01.