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. 2004 Nov;78(22):12694–12697. doi: 10.1128/JVI.78.22.12694-12697.2004

FIG. 2.

FIG. 2.

EBNA2ΔCR4 is transcriptionally competent in supporting LMP1 expression and Cp promoter activity. (A) Upper panel, Western blot probed with anti-EBNA2 PE2 monoclonal antibody (DAKO). ER-EBNA2 is expressed in EREB2-5 cells transduced with LK2. ER-EBNA2 plus EBNA2 are expressed in LK2-EBNA2- and LK2-EBNA2ΔCR4-transduced cells. Middle panel, Western blot showing loss of ER-EBNA2 expression in LK2-EBNA2 and LK2-EBNA2ΔCR4 cells grown continuously in the absence of estrogen. LK2-EREB2-5 cells maintained in estrogen-containing medium retain ER-EBNA2 expression. Lower panel, the membrane shown in the middle panel was stripped and reprobed with anti-LMP1 S12 monoclonal antibody (32a). LK2-EBNA2ΔCR4 cells showed no deficit in the ability to mediate LMP1 expression. (B) Transient expression assay in which HeLa cells were cotransfected with the EBV Cp promoter reporter 4× Cp-CAT and either control vector or an expression plasmid for EBNA2, the EBNA2 mutant (EBNA2WW), or EBNA2ΔCR4. EBNA2ΔCR4 was as effective as EBNA2 in activating expression of 4× Cp-CAT.