FIG. 1.

Complementation of a P-deficient RV in cells expressing eGFP-tagged P proteins. (A) Genome organization of SAD ΔP, lacking the entire P open reading frame, and the parental SAD L16. (B) Recruitment of cellular eGFP-tagged P proteins into RV inclusion bodies. Cells expressing the indicated P fusion proteins were infected with SAD ΔP or SAD L16. The appearance of fluorescent inclusion bodies at 1 day postinfection demonstrated nucleoprotein gene expression from SAD ΔP. Both eGFP-P and P-eGFP proteins also colocalized to inclusion bodies of wt SAD L16. In noninfected cells (n.i.), diffuse fluorescence was exclusively observed.