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Recovery of autonomously replicating RV expressing the eGFP-P fusion. (A) Genome organization of SAD L16 and SAD eGFP-P in which eGFP is fused to the N terminus of P. (B) Spread of SAD eGFP-P in noncomplementing BSR T7/5 infected at a MOI of 0.001 observed in live cells by fluorescence microscopy at the indicated time points. (C) Expression of eGFP-P in cells infected with SAD ΔP at a MOI of 1 after 2 days by Western blots with the indicated sera. n.i., not infected.
