FIG. 2.

The p53-Bax apoptotic pathway is not involved in CRAd(E3+)- or CRAd(E3−)-induced cell killing of H460 cells. Western blot analyses of p53 and Bax expression in H460 cells infected with CRAd(E3+) or CRAd(E3−) at an MOI of 25 at different time points after infection are shown. As a control, H460 cells were treated with CDDP or with FasL+CHX (Fas). β-Actin serves as a control for loading (A). The reduced expression level of p53 in stable HPV16-E6-overexpressing H460 cells (H460-E6) was confirmed (B). CRAd-induced cell killing at different MOIs was examined in H460-E6 cells and in empty vector-transfected control H460 cells (H460-neo) at 7 days after infection by WST-1 assays. The results obtained for CRAd(E3+) are shown and were similar to those for CRAd(E3−) (C). Values are means ± standard deviation of three experiments.