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. 2016 May 15;95:348–360. doi: 10.1016/j.watres.2016.03.037

Table 2.

Removal (in %) of seven selected micropollutants in cell-free lysates in dependence of experimental parameters. Only results ≥10% removal with significantly different removal rate constants from the negative control (prc < 0.05) are depicted. a substance was removed below its limit of quantification (see Table SI 1), b removal was <10%, slash (/) indicates that removal rate constants were not significantly different from negative control; n/a indicates that this substance was not spiked in the respective assay. US: ultrasonication, PF: pressure filtration, PI: peptidase inhibitors, AS: ammonium sulfate.

ID Category Condition Addition of PI Removal [%]
Acetaminophen Acetyl-SMX Atenolol Bezafibrate Codeine 10-OH-CBZ Erythromycin
1 Basic lysate Sludge A >85a 56 ± 4 24 ± 2 19 ± 4 / / /
2 Basic lysate Sludge B >85a 64 ± 1 31 ± 3 24 ± 4 / 25 ± 4 /
3 Basic lysate Sludge C >85a 56 ± 2 24 ± 5 18 ± 6 / / /
Mean (basic lysates 1–3) >85a 59 ± 5 26 ± 6 20 ± 8 / / /
4 Basic lysate Freeze/thaw >85a 59 ± 6 22 ± 1 21 ± 10 / / /
5 Pre-treatment +EDTA x >85a 74 ± 4 17 ± 8 10 ± 7 / / /
6 Pre-treatment −EDTA x >85a 74 ± 4 18 ± 6 b / / /
7 Pre-treatment +EDTA/US/PF x >85a 74 ± 1 / / / / /
8 Additives PI x >85a 34 ± 3 / / / / /
9 Additives AS x >85a >94a b b / / /
10 Lysis method Bead beating >85a 46 ± 5 28 ± 4 34 ± 2 b / /
11 Filtration No prefilter >85a 62 ± 4 42 ± 5 31 ± 6 / / >96a
12 Filtration Unfiltered >85a 53 ± 8 54 ± 16 25 ± 14 / / >96a
13 Analyte mix Reduced >85a n/a 31 ± 8 n/a / n/a n/a
14 Spike Low >85a 67 ± 11 22 ± 6 23 ± 4 / / /
15 Spike High >85a 58 ± 5 29 ± 4 28 ± 2 / / /
16 Cofactor NADH >85a 37 ± 2 b b / / /