Table 2.
Removal (in %) of seven selected micropollutants in cell-free lysates in dependence of experimental parameters. Only results ≥10% removal with significantly different removal rate constants from the negative control (prc < 0.05) are depicted. a substance was removed below its limit of quantification (see Table SI 1), b removal was <10%, slash (/) indicates that removal rate constants were not significantly different from negative control; n/a indicates that this substance was not spiked in the respective assay. US: ultrasonication, PF: pressure filtration, PI: peptidase inhibitors, AS: ammonium sulfate.
| ID | Category | Condition | Addition of PI | Removal [%] |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Acetaminophen | Acetyl-SMX | Atenolol | Bezafibrate | Codeine | 10-OH-CBZ | Erythromycin | ||||
| 1 | Basic lysate | Sludge A | >85a | 56 ± 4 | 24 ± 2 | 19 ± 4 | / | / | / | |
| 2 | Basic lysate | Sludge B | >85a | 64 ± 1 | 31 ± 3 | 24 ± 4 | / | 25 ± 4 | / | |
| 3 | Basic lysate | Sludge C | >85a | 56 ± 2 | 24 ± 5 | 18 ± 6 | / | / | / | |
| Mean (basic lysates 1–3) | >85a | 59 ± 5 | 26 ± 6 | 20 ± 8 | / | / | / | |||
| 4 | Basic lysate | Freeze/thaw | >85a | 59 ± 6 | 22 ± 1 | 21 ± 10 | / | / | / | |
| 5 | Pre-treatment | +EDTA | x | >85a | 74 ± 4 | 17 ± 8 | 10 ± 7 | / | / | / |
| 6 | Pre-treatment | −EDTA | x | >85a | 74 ± 4 | 18 ± 6 | b | / | / | / |
| 7 | Pre-treatment | +EDTA/US/PF | x | >85a | 74 ± 1 | / | / | / | / | / |
| 8 | Additives | PI | x | >85a | 34 ± 3 | / | / | / | / | / |
| 9 | Additives | AS | x | >85a | >94a | b | b | / | / | / |
| 10 | Lysis method | Bead beating | >85a | 46 ± 5 | 28 ± 4 | 34 ± 2 | b | / | / | |
| 11 | Filtration | No prefilter | >85a | 62 ± 4 | 42 ± 5 | 31 ± 6 | / | / | >96a | |
| 12 | Filtration | Unfiltered | >85a | 53 ± 8 | 54 ± 16 | 25 ± 14 | / | / | >96a | |
| 13 | Analyte mix | Reduced | >85a | n/a | 31 ± 8 | n/a | / | n/a | n/a | |
| 14 | Spike | Low | >85a | 67 ± 11 | 22 ± 6 | 23 ± 4 | / | / | / | |
| 15 | Spike | High | >85a | 58 ± 5 | 29 ± 4 | 28 ± 2 | / | / | / | |
| 16 | Cofactor | NADH | >85a | 37 ± 2 | b | b | / | / | / | |