FIG. 3.

Interaction of Nsp15 with DNA and heparin. (A) PhosphorImage of 7.5 M urea-20% polyacrylamide gel showing 5′-end-labeled DNA oligonucleotide (D23.2) incubated in the presence (+) or absence (−) of Nsp15 under standard assay conditions. Each treatment was done in triplicate. (B) Effects of heparin and DNA on cleavage of R10.2 RNA by Nsp15. R10.2 digestion was performed in the absence or presence of various concentrations of either heparin or DNA (indicated above the bars). After the RNAs were resolved on urea-polyacrylamide gels, uncleaved R10.2 was quantitated and shown as a percentage [FL (%)]. Substrate RNA incubated in the absence (−) of Nsp15 was set at 100%. Each error bar represents 1 standard deviation from the mean for each treatment.