TABLE 1.

Oligonucleotides and RNAs used in this study

Oligonucleotide or RNA	Orientation	Use	Sequence
DNA oligonucleotides
D23.3	5′	PCR-SARS-CoV	5′ CATATGAGTTTAGAAAATGTGGC 3′
D29.2	3′	RT-PCR, SARS-CoV	5′ GGATCCTTATTGTAGTTTTGGGTAGAAGG 3′
D27.2	5′	PCR, IBV	5′ ACATATGTCTATCGACAATATTGCTTA 3′
D27.3	3′	RT-PCR, IBV	5′ ACTCGAGTTGAAGCTGTGGATAACATG 3′
D27.4	5′	PCR, MHV	5′ ACATATGAGTTTAGAAAATGTAGTGTA 3′
D27.5	3′	RT-PCR, MHV	5′ ACTCGAGCTGCAAACGAGGATAGAAAG 3′
Chemically synthesized RNAs
R10.2		Nsp15 substrate	5′ UGGGAGUAUA 3′
R12		Nsp15 substrate	5′ CCCCCGCCCCCC 3′
SK21		Nsp15 substrate	5′ GACGCUGAAUUAGGACAUAGA 3′
LE19P		Nsp15 substrate	5′ UGUUAUAAUUAUUGUAUAC-puromycin 3′
F16.2		Nsp15 substrate	5′ fluorescein-GGUCAGGAUACCUGGC 3′
LE21		Nsp15 substrate	5′ UGUUAUAAUUAUUGUAUAUAC 3′
T7 RNA polymerase transcript SL13		Nsp15 substrate	5′ GGUGCAUAGCACC 3′
Double-stranded RNA ds34		Nsp15 substrate	5′ UGUUAUAAUUAUUGUAUAUACAAUAAUUAUAACU 3′
			3′ ACAAUAUUAAUAACAUAUAUGUUAUUAAUAUUGA 5′