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. 2004 Nov;78(22):12268–12276. doi: 10.1128/JVI.78.22.12268-12276.2004

FIG. 5.

FIG. 5.

(a to d) The nectin1-EGFR1 and nectin1-GPI chimeras serve as HSV receptors. Micrographs show J-nectin1-EGFR1 cells (a), J-nectin1-GPI cells (b), J-nectin1 cells (c), and receptor-negative J cells (d) infected with R8102 and stained with X-Gal. Note that J cells expressing the chimeric receptors are susceptible to HSV infection, while J cells are not. (e to h) Treatment of J-nectin1-GPI cells with PI-PLC reduces HSV infectivity. J-nectin1 cells (e and f) and J-nectin1-GPI cells (g and h) were mock treated (e and g) or treated with PI-PLC (f and h), infected with R8102, and stained with X-Gal at 6 h after infection. The treatment specifically reduced infectivity in J-nectin1-GPI cells but not in J-nectin1 cells. (i) One-step growth of R8102 in J-nectin1, J-nectin1-EGFR1, and J-nectin1-GPI cells. Cells were infected at 25 PFU/cell. Progeny virus was harvested at 24 h after infection and titrated on Vero cells.