TABLE 1.
Distribution of mutations within N, P, M, and G protein open reading framesa
| Nucleotide no.b | Corresponding proteins (amino acid position) | Nucleotide change and corresponding amino acid change in virus:
|
|||
|---|---|---|---|---|---|
| K25 | P25 | A15 | A25 | ||
| 163 | N (33) | A→G, Lys→Glu | A→G, Lys→Glu | ||
| 187 | P (141) | C→U, Thr→Met | C→U, Thr→Met | C→U, Thr→Met | |
| 2257 | M (3) | C→U, Ser→Phe | C→U, Ser→Phe | C→U, Ser→Phe | |
| 2937 | M (248) | U→C + U, Stop→Arg | |||
| 3421 | G (115) | C→A, Thr→Lys | C→A, Thr→Lys | C→A, Thr→Lys | |
| 3978 | G (301) | C→A, Gln→Lys | C→A + C, Gln→Lys | C→A + C, Gln→Lys | |
| 4122 | G (349) | G→U, Val→Phe | G→U, Val→Phe | G→U, Val→Phe | |
| 4139 | G (354) | A→U, Pro | |||
| 4434 | G (453) | U→G, Phe→Cys | |||
a
Mutations were analyzed after 25 passages in acutely infected BHK-21 cells (K25) and persistently infected LL-5 cells (P25) and 15 and 25 passages alternating between acute infection in BHK-21 cells and persistence in LL-5 cells (A15 and A25).
b
The nucleotide changes correspond to the sequence of the positive strand.