TABLE 1.

Nucleotide sequence variation of envelope and capsid genes from naturally infected mosquitoes and dengue patients

Source	Envelope gene			Capsid gene
	No. of clones	No. of substitutions/total no. of nucleotides	Meanc diversity (%)	No. of clones	No. of substitutions/total no. of nucleotides	Meanc diversity (%)
Mosquitoesa	21e	17/8,253	0.21	21	6/6,678	0.09
Patientsb
ID7	10	6/3,930	0.15	10	6/3,180	0.19
ID8	11	17/4,323	0.39	10	6/3,180	0.19
ID9	11e	20/4,323	0.46	10	8/3,180	0.25
ID15	10	17/3,930	0.43	10d,f	13/3,180	0.41
ID18	15d	21/5,895	0.36	10	6/3,180	0.19
ID20	11d	15/4,323	0.35	10	4/3,180	0.13
ID23	10	11/3,930	0.28	10	8/3,180	0.25
ID24	10	23/3,930	0.59	10	7/3,180	0.22
Overall	88	130/34,584	0.38	80	58/25,440	0.23

^aThe dengue virus sequences in mosquitoes were derived from homogenates of a pool of 22 female A. aegypti mosquitoes.

^bThe patients included four DF (ID7, ID8, ID9, and ID15) and four DHF (ID18, ID20, ID23, and ID24) cases according to the World Health Organization definition (30).

^cThe mean diversity is the number of substitutions divided by the total number of nucleotides sequenced.

^dOne clone contained a stop codon.

^eOne clone contained a single-nucleotide deletion.

^fTwo clones contained single-nucleotide deletions (one each).