TABLE 2.

Nucleotide sequence variation of envelope and capsid genes from experimentally infected mosquitoes

Source	Envelope gene			Capsid gene
	No. of clones	No. of substitutions/total no. of nucleotides	Meanb diversity (%)	No. of clones	No. of substitutions/total no. of nucleotides	Meanb diversity
Mosquitoesa
M1	11	3/4,323	0.07	NDd	ND	ND
M2	10	4/3,930	0.10	10	1/3,180	0.03
M3	10c	2/3,930	0.05	10	0/3,180	0
M4	9	5/3,537	0.14	10	6/3,180	0.19
M5	10	4/3,930	0.10	10	6/3,180	0.19
Overall	50	18/19,650	0.09	40	13/12,720	0.10

^aDengue virus sequences were derived from each A. aegypti mosquito after intrathoracic injection of dengue virus and confirmation by an immunofluorescent antibody test (1, 23).

^bThe mean diversity is the number of substitutions divided by the total number of nucleotides sequenced.

^cOne clone contained a single-nucleotide deletion.

^dND, not done.