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Virus release by MLV NC mutants. MLV Pr65Gag and p30CA released from transfected cells into culture fluid in pelletable form (V) and Pr65Gag in cell lysates (C) are analyzed by immunoblotting with an anti-p30CA antiserum. Lanes 1 and 2, wild type; lanes 3 and 4, C39S; lanes 5 and 6, NC(Δ16-23); lanes 7 and 8, PR−; lanes 9 and 10, C17-; lanes 11 and 12, C37-; lanes 13 and 14, C60-; lane 15, lysate of mock-transfected cells.
