TABLE 1.
Viruses and vectors used in this studya
| Virus or vector | Expression of:
|
Reference or source | ||||||
|---|---|---|---|---|---|---|---|---|
| E3-12.5K | E3-6.7K | E3-gp19K | ADP | RIDα | RIDβ | E3-14.7K | ||
| rec700b | + | + | + | + | + | + | + | 51 |
| dl731 | − | + | + | + | + | + | + | 5 |
| dl701 | + | − | + | + | + | + | + | 4 |
| dl740 | + | − | + | + | + | + | + | This report |
| dl754c | + | − | − | + | + | + | + | 12 |
| dl704 | + | + | − | + | + | + | + | 4 |
| pm734.1 | + | + | + | − | + | + | + | 39 |
| dl799 | + | + | + | + | − | − | + | 13 |
| dl762 | + | + | + | + | + | + | − | 5 |
| dl309d | + | − | + | + | − | − | − | 17 |
| Ad/null | − | − | − | − | − | − | − | 43 |
| Ad/E3 | + | + | + | − | + | + | + | 43 |
| Ad/RID/14.7K | − | − | − | − | + | + | + | 43 |
| Ad/RID | − | − | − | − | + | + | − | 43 |
| Ad/14.7K | − | − | − | − | − | − | + | 43 |
| Ad/6.7K | − | + | − | − | − | − | − | This report |
Replication-competent viruses used in this study were rec700, dl731, dl701, dl740, dl754, dl704, pm734.1, dl799, dl762, and dl309. RD (EIA-negative) vectors used in this study were Ad/null, Ad/E3, Ad/RID/14.7K, Ad/RID, Ad/14.7K, and Ad/6.7K. The expression cassette, which consists of the cytomegalovirus promoter driving the expression of the indicated protein(s), is placed into the deleted E1A region. The natural E3 region is deleted in these vectors. −, no expression; +, expression.
The virus rec700 is an Ad5-Ad2-Ad5 recombinant and is considered to be the wt parent of virus mutants with “700” designations.
The mutant dl754 contains a deletion that removes the C-terminal coding sequence of E3-6.7K and the N-terminal coding sequence of gp19K.
The mutant dl309 is a derivative of Ad5. Sequence analysis showed that the E3-6.7K gene in dl309 contains a mutation (3) that renders the E3-6.7K protein nonfunctional (A. E. Tollefson, unpublished observations).