FIG. 3.
Superposition of WT and V82A complexes. (a) Stereo diagram of the superimposed active site residues in the WTNC-p1WT (in gray and cyan) and AP2VNC-p1V82A (in magenta and purple) complexes. Protease residues within 4.2Å of the substrate are shown in ball-and-stick representation, and residue 82 is highlighted in yellow. The substrate mutation site (P2) is circled, and regions exhibiting large structural changes are indicated with arrows. Similarly, panels b and c show the superposition of CA-p2 and p1-p6, respectively, in complex with the WT and V82A protease variants. Note that there are virtually no conformational changes seen between the WT and V82A complexes in these two complexes.