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The nNLS is essential for Kapβ2- or Kapβ3-mediated nuclear import of HPV16 L2. Digitonin-permeabilized HeLa cells were incubated with either wild-type L2 protein (panels A to C) or L2ΔN mutant protein (panels D to F) in the presence of either transport buffer (panels A and D), Kapβ2 plus Ran-GDP (panels B and E), or Kapβ3 plus Ran-GDP (panels C and F). Note the nuclear import in panels B and C.
