FIG. 6.

Deletion of both the N and C termini of HPV16 L2 abolishes interaction with the Kapα₂ adapter. Blots containing wild-type L2 protein (lanes 1 and 7), L2ΔN mutant protein (lanes 2 and 8), L2ΔM mutant protein (lanes 3 and 9), L2ΔC mutant protein (lanes 4 and 10), L2ΔNΔC double mutant protein (lanes 5 and 10), and L2ΔNΔMΔC triple mutant protein (lanes 6 and 12) were either probed with an anti-His antibody (lanes 1 to 6) or incubated with 2.5 μg of Kapα₂ per ml, and bound Kapα₂ was detected with an anti-Kapα₂ antibody (lanes 7 to 12).