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The nNLS of HPV16 L2 efficiently mediates the nuclear import of a GST reporter via the Kapα2β1 pathway. Digitonin-permeabilized cells were incubated with either GST-nNLS (panels A to C), GST-cNLS (panels D to F), or GST-NLSHPV16L1 (panels G to I) in the presence of either transport buffer (panels A, D, and G), Kapβ1 plus Ran-GDP (panels B, E, and H), or Kapα2 plus Kapβ1 plus Ran-GDP (panels C, F, and I). Nuclear import of the GST-NLSs was detected with an anti-GST antibody.
