TABLE 4.
Detection of adenoviruses and enteroviruses in river water samples by cell culture-RT-multiplex PCR assay
| Sampling site | Virus detection by cell culture-RT-multiplex PCR assay
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Group 1 (BGMK)
|
Group 2 (BGMK + A549)
|
||||||||
| Group 2A (BGMK)
|
Group 2B (A549)
|
Total (groups 2A and 2B)
|
|||||||
| Adenovirus | Enterovirus | Othersa | Adenovirus | Enterovirus | Adenovirus | Enterovirus | Adenovirus | Enterovirus | |
| Sanbon River | 1/10 (2/60)b,c | 8/10 (22/60) | 1/10 (1/60) | 0/10 (0/30) | 8/10 (15/30) | 6/10 (10/30) | 5/10 (12/30) | 6/10 (10/60) | 8/10 (27/60)f |
| Hwajeong River | 0/10 (0/60) | 4/10 (8/60) | 2/10 (2/60) | 0/10 (0/30) | 6/10 (8/30) | 3/10 (4/30)e | 7/10 (15/30)e | 3/10 (4/60) | 8/10 (23/60)f |
| Ansan River | 0/10 (0/60) | 3/10 (3/60) | 1/10 (1/60) | 0/10 (0/30) | 3/10 (4/30) | 2/10 (2/30) | 0/10 (0/30) | 2/10 (2/60) | 3/10 (4/60) |
| Siheung River | 0/10 (0/60) | 4/10 (4/60)d | 2/10 (2/60) | 0/10 (0/30) | 0/10 (0/30) | 2/10 (3/30) | 3/10 (5/30) | 2/10 (3/60) | 3/10 (5/60) |
| Total | 1/40 (2/240) | 19/40 (37/240) | 6/40 (6/60) | 0/40 (0/120) | 17/40 (27/120) | 13/40 (19/120) | 15/40 (32/120) | 13/40 (19/240) | 22/40 (59/240) |
a
Samples exhibited CPE, but the cell culture-RT-multiplex PCR results were negative.
b
No. of positive samples/no. of samples tested. (No. of positive subsamples/no. of subsamples tested).
c
Two subsamples obtained on 13 June 2002 did not exhibit CPE but were positive for adenoviruses as determined by a nested PCR.
d
One subsample obtained on 28 June 2002 that did not exhibit CPE was positive for enteroviruses as determined by a nested PCR.
e
One subsample obtained on 13 June 2002 was coinfected with both adenoviruses and enteroviruses.
f
Total number of positive samples detected in either group 2A or group 2B.