FIG. 6.

Evolution of the hepatotoxicity in cyanobacterial genera by using phylogenetic characterization of the ABC transporter and 16S rRNA genes. All sequences were obtained from GenBank, and the accession numbers are given in parentheses. The sequence alignment and phylogenetic tree were created by using Pileup and ClustalX from GCG (40, 48). The scale bar represents two substitutions per 100 nucleotides. Significant bootstrap values calculated from 1,000 bootstrap trees are indicated at the nodes (11). A) Phylogenetic analysis of the putative ABC transporter gene. The ndaI gene from N. spumigena NSOR10 was aligned with the mcyH sequences from M. aeruginosa strains PCC7806 and K-139 and from P. agardhii CYA126/8 and with the putative ABC transporter nosG sequence from Nostoc sp. strain GSV224. B) Phylogenetic analysis of the 16S rRNA gene.