Fig. 5. Nrf2 inactivation reduces hepatic GSH reserve.

(A) Hepatic GSH levels in wild-type (wt) and sibling nrf2^fh318/fh318 (nrf2) larvae normalized to wt controls. Data represent mean of three experiments with five larvae per condition for each experiment. Error bars, SEM. *p < 0.03 compared to wt control larvae. (B) nCounter analysis of Nrf2 target gene expression in livers of wt and nrf2 mutant larvae with and without biliatresone treatment. Data represent mean of three experiments with 30 larvae dissected per condition for each experiment. Error bars, SEM. *p < 0.03 in comparison to DMSO-treated controls, **p < 0.03, wt versus nrf2 mutants. Abbreviations: nrf2, nrf2^fh318/fh318; wt, wild-type.