TABLE 1.

Sequences of primers used for seminested PCRs

Primera	Sequence (5′-3′)	Seminested PCR round
C142f	AAABGRMKRYTAATACCGCATAA	1st
Cy5-C142f	Cy5-AAABGRMKRYTAATACCGCATAA	2nd
C1090r	TRGCWACTAASRATAAGGG	1st
C796r	CCCNACACCTAGTAYYCATC	2nd

^aIn accordance with the 16S rRNA gene sequences of 40 strains of plant clostridia (accession numbers AB114225 to 64), primers C142f (Cy5-C142f) and C1090r were designed by the modification of S-*-chis-0150-a-S-23 (6) and S-*-Ccoc-1112-a-A-19 (22), respectively.