Fig. 3.

Sorting nexin-4 (SNX4) expression increases β-site amyloid precursor protein-cleaving enzyme 1 (BACE1) levels and β-amyloid (Aβ). a HEK293 cells were cotransfected with BACE1 and either mock or SNX4. Quantitative Western blot analysis was performed using anti-BACE1, anti-β-actin, anti-Aβ (6E10), anti-amyloid precursor protein (anti-APP), anti-soluble ectodomain-secreted β-amyloid precursor protein derivative (anti-sAPPβ), and anti-SNX4 antibodies. The amount of Aβ was analyzed in the culture media by immunoblotting. b Quantification of Western blot band intensities. The graphs display the immunoreactivity to anti-BACE1, anti-APP, anti-sAPPβ, and anti-Aβ antibodies, normalized to β-actin. **p < 0.01, ***p < 0.001. c HeLa cells were cotransfected with BACE1-hemagglutinin (HA) and either mock or SNX4. Immunocytochemistry was performed using an anti-HA antibody (green). Scale bar = 20 μm. d Primary mouse cortical neurons were transfected with either mock green fluorescent protein (GFP) or SNX4-GFP. Quantitative Western blot analysis was performed using anti-BACE1, anti-Aβ (6E10), anti-APP, anti-sAPPβ, anti-β-actin, and anti-GFP antibodies. The amounts of Aβ and sAPPβ were analyzed in the culture medium by immunoblotting. e Quantification of Western blot band intensities. The graphs display the immunoreactivity to BACE1, APP, sAPPβ, and Aβ antibodies, normalized to β-actin. **p < 0.01, ***p < 0.001. f Primary neurons were cotransfected with BACE1-HA and either mock or SNX4. Immunocytochemistry was performed using an anti-HA antibody (green). Scale bar = 20 μm