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. 2004 Nov;70(11):6875–6883. doi: 10.1128/AEM.70.11.6875-6883.2004

FIG. 1.

FIG. 1.

A. flavus contains two GGPP synthases. (A) P. paxilli paxG gene map showing conserved domains (I to V), intron positions (inverted triangles), and consensus polypeptide sequences used to design degenerate primers to amplify the region between domains III and V. (B) PCR products amplified with degenerate primers ggpps27 and ggpps29 (lanes 2 and 4) and ggpps27 and ggpps28 (lanes 3 and 5) from genomic DNAs of P. paxilli (lanes 2 and 3) and A. flavus (lanes 4 and 5). The products shown in lanes 3 and 5 were from nested PCR amplifications of 1/100 dilutions of the products shown in lanes 2 and 4, respectively. Lane 1 contains a 1-kb DNA ladder (Invitrogen).