TABLE 1.
Primer | Sequence (5′→3′)a |
---|---|
ggpps27 | CAYMGIGGTCARGGTATGGA |
ggpps28 | TTCATRTAGTCGTCICKTATYTG |
ggpps29 | AACTTTCCYTCIGTSARGTCYTC |
M13F | CCCAGTCACGACGTTGTAAAACG |
M13R | AGCGGATAACAATTTCACACAGG |
T7 | TAATACGACTCACTATAGGG |
Oligo-dT | GAGAGAATTCGGATCCTCTAGAG(T)20 |
AF-93 | ACGGATCCAGGAGTGGCGTTATCGTG |
AF-94 | ACGGATCCAATAGGAGAGGTTAGTCG |
AF-tub-1 | CTTCTTCATGGTTGGCTTCG |
AF-tub-2 | CTCTGATCTTCGCAAGTTGG |
AF-tub-3 | GGTGGAGGACATCTTGAGAC |
AF-111 | CCTTCTACGTCTCCATCCAG |
AF-112 | GTGATCTCCTTCTGCATACG |
atmGF | TAGTCGTCACGGATCTGGAA |
atmGR | CAAGTTCATTGTCGACCGAG |
atmMF | GGCATCAATGGCATTATCC |
atmMR | GGACAATGTATCGTGCAAGG |
AF-78 | AGGACAGGCTGAACATCATC |
AF-79 | GTTATCCGCTCTGAGATGTG |
AF-80 | CTGTCATAGACGTAACCTCC |
AF-81 | TCTCTTCGAGACATTGCAGC |
atmCF | TCGGATATTATGTGGCGACC |
atmCR | GTTGCCGCCTCTGTTGCCTT |
AF-81 | TCTCTTCGAGACATTGCAGC |
AF-82 | TTCAACCAGAGGAGCGAGTA |
AF-106 | CTTAGTCAGCCTCGCTATAC |
AF-113 | GATTACAAGGTGAGTGGAGC |
AF-114 | GCTCCACTCACCTTGTAATC |
AF-115 | AGAGGAAGTCCTACCGCTCA |
AF-116 | TGAGCGGTAGGACTTCCTCT |
AF-117 | GGATCAGAGCCGGTGAGACA |
AF-9 | GAAGACCGAGCACATTGA |
AF-108 | GTATGTCCTCTTGCATGTCC |
I, inosine; M, A or C; K, G or T; R, A or G; S, C or G; and Y, C or T. Italic bases do not correspond to the A. flavus genomic sequence.