TABLE 2.

CVs of virus assays described^a

PCR target	Quantity	Intra-assay variation		Interassay variation (CV2)
		CV1a	CV1b
CMV	1.00E+05	0.22	0.21	2.41
	1.00E+04	0.94	1.09	2.12
	1.00E+03	0.57	0.47	1.90
	1.00E+02	0.80	0.26	1.56
	1.00E+01	0.38	2.12	1.99
EBV	1.00E+06	0.30	0.63	2.96
	1.00E+05	1.15	0.80	2.80
	1.00E+04	0.32	0.28	1.90
	1.00E+03	0.24	0.17	1.83
HHV-6	1.00E+06	0.27	0.39	1.42
	1.00E+05	0.26	0.52	1.63
	1.00E+04	0.51	0.50	1.00
	1.00E+03	0.08	0.63	0.84
	1.00E+02	0.25	1.56	1.60
HHV-7	1.00E+05	0.85	0.41	2.45
	1.00E+04	0.11	0.26	1.89
	1.00E+03	0.45	0.21	1.69
	1.00E+02	0.50	0.26	1.73
	1.00E+01	0.20	0.28	1.24
HHV-8	1.00E+06	0.52	0.46	1.07
	1.00E+05	0.29	0.44	0.77
	1.00E+04	0.53	0.11	0.82
	1.00E+03	0.68	0.58	0.81
	1.00E+02	0.23	0.20	0.67
HSV-1b	1.00E+05	0.38	0.10	2.60
	1.00E+04	0.30	0.20	1.99
	1.00E+03	0.20	0.19	1.79
	1.00E+02	0.51	0.22	1.61
HSV-2	1.00E+05	2.42	0.62	2.05
	1.00E+04	0.37	0.12	1.80
	1.00E+03	0.87	0.36	1.73
	1.00E+02	0.33	0.36	1.43
VZV	1.00E+05	0.64	0.46	3.67
	1.00E+04	0.17	0.20	3.09
	1.00E+03	0.39	0.38	3.17
	1.00E+02	0.29	0.13	2.69
	1.00E+01	2.94	1.15	3.91
AdV A	1.00E+06	0.32	0.62	1.16
	1.00E+05	0.18	0.24	1.08
	1.00E+04	0.18	0.37	0.85
	1.00E+03	0.40	0.17	0.84
AdV B	1.00E+05	0.32	0.07	1.92
	1.00E+04	0.34	0.06	1.79
	1.00E+03	0.39	0.26	1.73
	1.00E+02	0.23	0.17	1.27
AdV C	1.00E+05	0.50	0.51	0.56
	1.00E+04	0.40	0.31	0.75
	1.00E+03	0.07	0.15	0.45
	1.00E+02	0.21	0.22	0.43
	1.00E+01	0.75	0.81	0.73
AdV D	1.00E+05	0.53	0.68	1.79
	1.00E+04	0.09	0.45	1.92
	1.00E+03	0.37	0.15	1.49
	1.00E+02	0.42	0.11	1.08
AdV E	1.00E+05	0.18	0.57	1.17
	1.00E+04	0.17	0.15	0.78
	1.00E+03	0.63	0.43	0.74
	1.00E+02	0.32	0.33	0.50
	1.00E+01	0.45	0.58	1.34
AdV F	1.00E+06	0.13	0.30	0.87
	1.00E+05	0.19	0.32	1.17
	1.00E+04	0.22	0.15	1.02
	1.00E+03	0.13	0.27	0.82
BKV	3.00E+06	0.31	0.21	1.02
	3.00E+05	0.36	0.46	1.08
	3.00E+04	0.22	0.27	0.86
	3.00E+03	0.33	0.39	0.96
	3.00E+02	0.25	0.16	0.79
JCV	1.60E+05	0.21	0.18	0.53
	1.60E+04	0.53	0.30	0.56
	1.60E+03	0.33	0.27	0.52
	1.60E+02	0.30	0.24	0.35
	1.60E+01	0.61	1.15	0.83
PVB19	1.00E+06	0.56	0.57	2.33
	1.00E+05	0.35	0.37	2.49
	1.00E+04	0.38	0.25	2.41
	1.00E+03	0.43	0.37	2.07
Enterovirus	1.00E+07	0.24	0.45	1.58
	1.00E+06	0.43	0.44	1.31
	1.00E+05	0.63	0.40	1.18
	1.00E+04	0.62	0.42	1.08
	1.00E+03	1.75	2.57	2.39
RSVc	1.00E+05	0.67	0.39	3.20
	1.00E+04	0.55	0.21	2.06
	1.00E+03	0.39	0.40	1.66
	1.00E+02	0.58	0.59	2.43
Influenza A virus	1.00E+06	0.34	0.25	2.52
	1.00E+05	0.34	0.20	1.99
	1.00E+04	0.83	0.50	1.78
	1.00E+03	0.60	0.22	1.31
Influenza B virus	1.00E+04	0.70	0.73	2.78
	1.00E+03	0.30	0.31	2.36
	1.00E+02	0.39	0.40	2.10
	1.00E+01	1.12	1.16	2.13
PIV-1d	1.00E+05	0.88	0.26	1.50
	1.00E+04	0.18	0.19	1.46
	1.00E+03	0.43	0.09	1.30
	1.00E+02	0.34	0.21	1.20
	1.00E+01	0.52	0.32	1.03
PIV-2	1.00E+05	0.06	0.24	1.84
	1.00E+04	0.41	0.19	1.22
	1.00E+03	0.17	0.29	1.33
	1.00E+02	0.18	0.19	1.20
	1.00E+01	0.73	0.94	1.25
PIV-3	1.00E+04	0.32	0.26	0.57
	1.00E+03	0.82	0.57	0.72
	1.00E+02	0.55	0.50	0.59
	1.00E+01	0.44	1.56	1.29

^aCVs observed in RQ-PCR analyses of different virus quantities are presented. The intra-assay variation was calculated by comparing the C_t values of two simultaneously amplified triplicate reactions (CV1a versus CV1b). The interassay variation was assessed by comparing triplicate reactions of different RQ PCR runs (CV2). The mean values for intra-and interassay variation across all virus assays are as follows: CV1a, 0.46; CV1b, 0.43; CV2, 1.55.

^bHSV-1, herpes simplex virus type 1.

^cRSV, respiratory syncytial virus.

^dPIV-1, parainfluenza virus type 1.