TABLE 2.
Primers used for PCR, DNA sequencing, and AFLP genotyping
| Primer | Locus | Sequence (5′ to 3′)a |
|---|---|---|
| EF-1 | EF-1α | ATGGGTAAGGARGACAAGAC |
| EF-11ta | EF-1α | GTGGGGCATTTACCCCGCC |
| EF-22t | EF-1α | AGGAACCCTTACCGAGCTC |
| EF-2 | EF-1α | GGARGTACCAGTSATCATG |
| MS1 | mtSSU rDNA | CAGCAGTCAAGAATATTAGTCAATG |
| MS2 | mtSSU rDNA | GCGGATTATCGAATTAAATAAC |
| MS21 | mtSSU rDNA | CTCTCCTCCTCAAGTACTGC |
| GFM136 | MAT1-1 | ATGGTCTACAGCCAGTCGCA |
| FOM132 | MAT1-1 | GGTAGTGTTGTTTGTGGTTG |
| FOM122 | MAT1-1 | TCCATGCCAAGATCCTCAGC |
| FOM123 | MAT1-1 | AAGGCAGAGTCAGAAATCCA |
| FOM112 | MAT1-1 | GCTGCTGCATCTTGGATTGC |
| FOM111 | MAT1-1 | GCTTGATCTGTTCGGTCATG |
| FOM211 | MAT1-2 | ACATATCGATAGCATCTACC |
| FOM212 | MAT1-2 | AGGCGGTAATCTGCTGTGTA |
| NL11 | IGS rDNA | CTGAACGCCTCTAAGTCAG |
| OCNL13 | IGS rDNA | TGTGATGTATGCGGTCCTAGG |
| ONL13B | IGS rDNA | GGTTCGAGGATCGATTCGAGG |
| OCNS3C | IGS rDNA | GCAAGATCTGATACTGAGAGG |
| CNS1 | IGS rDNA | GAGACAAGCATATGACTAC |
| EcoRI-a | Adapter | CTCGTAGACTGCGTACC |
| EcoRI-b | Adapter | AATTGGTACGCAGTCTAC |
| MseI-a | Adapter | GACGATGAGTCCTGAG |
| MseI-b | Adapter | TACTCAGGACTCAT |
| EcoRI-ns | Nonselective | GACTGCGTACCAATTC |
| MseI-ns | Nonselective | GATGAGTCCTGAGTAA |
| EcoRI-CC | Selective | 6-FAM-GACTGCGTACCAATTCCC |
| EcoRI-TC | Selective | 6-FAM-GACTGCGTACCAATTCTC |
| MseI-G | Selective | GATGAGTCCTGAGTAAG |
a
R, AG; S, CG.