FIG. 5.

Induction of linE- and linD-specific mRNAs after addition of different HCHs. At time zero a single dose of HCH was added to the chemostat culture of S. paucimobilis B90A. (A and B) α-HCH (filled symbols) and δ-HCH (open symbols) at concentrations of 2 and 20 mg per liter, respectively. (C and D) γ-HCH (0.7 mg/liter) and β-HCH (5 mg/liter). The corresponding autoradiogram used for scanning is included within the graphs. The mRNA amounts are represented as the mean DNA equivalent copy number (by comparing to a DNA standard, as explained in Materials and Methods) per microgram of RNA loaded in each spot. Induction experiments with 0.7 mg of γ-HCH per liter were carried out twice; other experiments were carried out once.