TABLE 1.

Bacterial strains, plasmids, and primers used in this study

Strain, plasmid, or primer	Relevant characteristic(s)a or nucleotide sequenceb	Source, reference, or relative position of primer
Bacterial strains
E. coli DH10B	Plasmid-free strain used for cloning	13
E. coli K12	Reference strain	DSM498
S. oneidensis MR-1	Lake Oneida, N.Y., sediment	35
S. oneidensis DLM7	Lower Green Bay sediment, Lake Michigan, Wis.	52
S. oneidensis MR-4	Black sea water column, 5 m	52
S. putrefaciens 200	Crude oil pipeline, Canada	ATCC
S. algae	Red algae, Japan	ATCC
P. aeruginosa PAO1	Prototrophic, no UV resistance plasmid present	A. M. Chakrabarty
P. aeruginosa UA11079	Same as PAO1, but Rifr, uvrA::ΩHg (Hgr), plasmid-free strain	39
Plasmids
pJJK20	Apr, E. coli umuDC promoter source	24
pJB321	Cbr, broad-host-range cloning vector	3
pRK2013	Helper plasmid for triparental matings	9
pXQ01	Apr, cloned MR-1 uvrA coding sequence as NdeI-BamHI in pJJK20	This study
pXQ03	Apr, umuDC promoter and MR-1 uvrA coding sequence from pXQ01 as SphI-BamHI in pJB321	This study
Primersc
uvrA NdeI 5′	GATCCATATGGATAAGATTGAAATACGCGGTGC	ATG start codon
uvrA BamHI 3′	GATCGGATCCCTACTGCTGTTTGGTTAGC	CTA stop codon
uvrA1F	TAACGGTCGTAAGGGTGAGC	465
uvrA1R	GAGAGTTCGAGTGCGGTTTC	683
uvrA2F	GCTTATTCACCTGCGTGACA	1898
uvrA2R	GTATAAGTGGCGGGGTTTGA	2114
uvrB1F	CACCATCGCCAATGTGATAG	144
uvrB1R	CAATCAGCACCACATCCTTG	421
uvrB2F	TAGTGCCTAAAGGGGTGGTG	1754
uvrB2R	CCCTTAAGCGTTTCACCTCA	2002
uvrD1F	AAGACCAAGGATTACGGCCT	449
uvrD1R	ATCCAAGCGTATTGAATGGC	698
uvrD2F	CAAGAGCTCACGTTATGGCA	1297
uvrD2R	CTCTTCAGGCATTTCGAAGG	1572

^aPhenotype resistance (r) abbreviations are: Ap, ampicillin; Cb, carbenicillin; Hg, mercury; Rif, rifampin.

^bFor primer oligonucleotide sequences, the restriction sites incorporated in primers are underlined. CATATG, NdeI; GGATCC, BamHI.

^cPrimers were designed using putative gene sequences of S. oneidensis MR-1.