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. 2004 Nov;70(11):6587–6594. doi: 10.1128/AEM.70.11.6587-6594.2004

FIG. 2.

FIG. 2.

Coomassie staining (A) and immunodetection (B and C) of Cry1Ac1 (lanes 1 to 4) and Cry1Ac1-D (lanes 5 to 8) expressed from plasmids pSVC7 and pSVC1-31A. Lanes 1, 3, 5, and 7 show the supernatant fraction of the cultures, and lanes 2, 4, 6, and 8 show the pellet fraction of cultures under noninduced conditions (lanes 1, 2, 5, and 6) and under induced conditions (lanes 3, 4, 7, and 8). Lanes M, molecular size markers. Panels B and C show immunodetection with anti-Cry1Ac1 and anti-D antibodies, respectively.