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Cry1Ac1 and Cry1Ac1-D ligand blot of brush border membrane vesicles samples. Lane M, molecular size markers (in kilodaltons). Lane 1: overlay of gut extract-treated Cry1Ac1 protein over brush border membrane vesicles (40 μg). Lane 2: overlay of gut extract-treated Cry1Ac1-D fusion protein over brush border membrane vesicles (40 μg). Lane 3: overlay of nontreated Cry1Ac1-D fusion protein over brush border membrane vesicles (40 μg).
