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Immunoblot detection of Cry1Ac1-D and Cry1Ac1-phage with anti-D (A) and anti-Cry1Ac1 (B) antibodies. Lane M: molecular size markers (in kilodaltons). Lanes 1: Cry1Ac1-D fusion protein expressed in E. coli BLR. Lanes 2: E. coli LE392 lysates obtained by infection with Vc phage. Lanes 3: E. coli LE392 lysates obtained by infection with V13 phage.
