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. 2004 Nov;70(11):6587–6594. doi: 10.1128/AEM.70.11.6587-6594.2004

TABLE 1.

Primers

Primer Sequence Restriction site underlined
SV1 5′-AAACGCGTCCCATTGAGAGG MluI
SV2 5′-ATGGGACGCGTTTCTTGTAC MluI
SV3 5′-AGTGGAGGTGGTGGATCAGGTGGAG GAGGT
SV4 5′-CTCCAGATTATATATTCAGCC
NT56 5′-CACCGCTGCGGCCGCTCTTTCCGATT ATATTC NotI
Ken72 5′-CACACAAGCTGTGACCGTC
SV9 5′-AAATGGATCCAGACGAAAGGG BamHI
SV12 5′-TTAGAGCGGCCGCAACAATTATTAG AAACATTAGC NotI
SV13 5′-GCTATGGATCCGCCTACTATTTTTG GTAGCATCCCC BamHI