TABLE 1.
Primer | Sequence | Restriction site underlined |
---|---|---|
SV1 | 5′-AAACGCGTCCCATTGAGAGG | MluI |
SV2 | 5′-ATGGGACGCGTTTCTTGTAC | MluI |
SV3 | 5′-AGTGGAGGTGGTGGATCAGGTGGAG GAGGT | |
SV4 | 5′-CTCCAGATTATATATTCAGCC | |
NT56 | 5′-CACCGCTGCGGCCGCTCTTTCCGATT ATATTC | NotI |
Ken72 | 5′-CACACAAGCTGTGACCGTC | |
SV9 | 5′-AAATGGATCCAGACGAAAGGG | BamHI |
SV12 | 5′-TTAGAGCGGCCGCAACAATTATTAG AAACATTAGC | NotI |
SV13 | 5′-GCTATGGATCCGCCTACTATTTTTG GTAGCATCCCC | BamHI |