FIG. 3.
Recovery of DNA from the QIAGEN columns used with the tissue kit and the stool kit. Three replicates of each type of water sample (ASW, PTS, or DWS) were spiked with two P. marinus cell concentrations, 100 (a) and 20 (b) cells/ml of water. After each of the five elutions was performed, the mean cell concentration per reaction mixture and the standard deviation were calculated on the basis of the P. marinus DNA concentration, the CT values, and the regression formulas for the PmarITS primers.