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. 2017 Jan 19;5:e2895. doi: 10.7717/peerj.2895

Figure 4. Jellyfish hexane extract induces apoptosis via extrinsic and intrinsic pathways in K562 cells.

Figure 4

(A) After treatment of K562 cells with various concentrations (0, 10, 20, 30, and 40 mg/ml) of Jellyfish-HE for 24 h, the expression levels of PARP, caspase-3, caspase-8 and caspase-9 were analyzed by immunoblotting with antibodies specific for PARP, caspase-3, caspase-8, and caspase-9. (B) The ratio of each protein to β-actin was calculated using Image J software. (C) After treatment with Jellyfish-HE at various concentrations for 24 h, levels of Bcl-2 and BAX proteins were analyzed by immunoblotting and the BAX/Bcl-2 ratio was quantified by densitometry (D). K562 cells were treated with 40 mg/ml Jellyfish-HE for 12 h in the presence or absence of Z-VAD and then (E) cell viability was measured by an MTT assay and analyzed by immunoblotting (F) with antibodies specific for PARP and capase-3. b-Actin was used as a loading control. * P < 0.05, ** P < 0.005 and *** P < 0.0005 vs. control (untreated). # P < 0.05 vs. treatment with Jellyfish-HE.