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. 2017 Jan 19;5:e2895. doi: 10.7717/peerj.2895

Figure 5. Jellyfish hexane extract induces apoptosis through the p38 MAPK pathway.

Figure 5

pERK1/2, ERK, pJNK, JNK, p-p38, and p38 were specifically detected by immunoblotting analysis at time intervals of 0, 0.25, 0.5, 1, 1.5, and 2 h after treatment with 40 mg/ml Jellyfish-HE (A). After treatment with various concentrations (0, 10, 20, 30, and 40 µg/ml) of Jellyfish-HE for 12 h (C), cell extracts were prepared and separated on SDS-PAGE followed by Western blot analysis. Band densities were then analyzed by densitometry using Image J software (B) and (D). After treatment of the cells with 40 µg/ml Jellyfish-HE in the presence or absence of p38 inhibitor, SB203580, cell viability was measured by the MTT assay (E) and the levels of p-p38, pro caspase-3, and cleaved caspase-3 were specifically detected using Western blots with antibodies specific to p-p38 and caspase-3 (F). β-Actin was used as an internal control. * P < 0.05, ** P < 0.005 and *** P < 0.0005 vs. control (untreated). ## P < 0.005 vs. treatment with Jellyfish-HE.