TABLE 1.
Medium composition for A. eutrophus
| Component | Amt in medium no. 1 (g liter−1) | Component | Amt in:
|
|
|---|---|---|---|---|
| Medium no. 2 (g liter−1) | Medium no. 3 (g liter−1) | |||
| Yeast extract | 10 | Glucose | 10 | 20 |
| Polypeptone | 10 | (NH4)2SO4 | 1.0 | 4.0 |
| Meat/beef extract | 5 | MgSO4 · 7H2O | 0.2 | 1.2 |
| (NH4)2SO4 | 5 | KH2PO4 | 1.5 | 13.3 |
| Na2HPO4 · 12H2O | 9.0 | |||
| Citric acid | 1.7 | |||
| Trace element solutiona | 1.0 ml liter−1 | 10 ml liter−1 | ||
a
Trace element solution has the following composition (per liter): FeSO4·7H2O, 10 g; ZnSO4·7H2O, 2.25 g; CuSO4·5H2O, 1 g; MnSO4·H2O, 0.35 g; CaCl2·2H2O, 2 g; Na2B4O7·10H2O, 0.23 g; (NH4)6Mo7O24, 0.1 g; HCl, 35%, 10 ml. The pH of the medium was adjusted to 6.8 to 7.0 with 1.0 M NaOH. To make solid medium, 15 g of agar liter−1 was used.