FIG. 3.

Variations of the relative number of kinetoplast DNA minicircles among culture promastigotes and amastigotes in human samples. DNA from 13 L. infantum (a to m) and 3 L. donovani (n, o, p) culture promastigotes was tested (A). Samples a1 to a5 correspond to different in vitro passages of the same strain. For the amastigote stage (B), relative quantification was performed on positive biological samples from six patients (a to f); the number next to the patient designation indicates the delay (in months) between each sampling. The b0,e0, and f0 samples were from bone marrow, and the others were blood samples.