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. 2004 Nov;70(11):6686–6694. doi: 10.1128/AEM.70.11.6686-6694.2004

FIG. 2.

FIG. 2.

Biofilm-forming characteristics of KM'92 bioreactor-derived isolates. (A) Stainless steel colonization and isolation of morphotypic variants. Mean cell densities attached to the stainless steel coupons in bioreactors inoculated with the single morphotypic variant of KM'92 are indicated on the x axis. The morphologies of the resultant colonies are indicated: wild type (Wt), black; SR, grey; LR, white; RS, striped. Viable cells were estimated by the swabbing technique and plating serial dilutions onto TSAYE. Standard deviations were calculated from mean viable cell numbers from triplicate coupons. (B) Visible cell growth (indicated by the arrow) emanating from the surface of stainless steel coupons within a bioreactor inoculated with KM'92 morphotype LR. Bar, 0.75 cm. (C) Epifluorescence microscopy of L. monocytogenes cells attached to the surface of a stainless steel coupon. The coupon was removed from a bioreactor inoculated with the LR morphotype. Inset: Colonization of a stainless steel surface from a bioreactor inoculated with the wild-type morphotype. Bar. 10 μm.