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Primer sequences used to detect gfbA and rofA in GGSa
| Primer | Sequence |
|---|---|
| Forward primers | |
| sfbI-F1 | 5′-GTCTTTCTTGACAATAACGTGGTAAGCTC-3′ |
| rofA-F | 5′-GCCAATAACTGAGGTAGC-3′ |
| Reverse primers | |
| sfbI-R3 | 5′-GTATCTTCAACAATGGTCACTGTTTCACTG-3′ |
| sfbI-R6 | 5′-GCAGCATAGGCTACTTGACCAAAAC-3′ |
| rofA-R | 5′-GGTTTTGCTCTTTTAGGT-3′ |
The primer locations within the genes and PCR strategy were described previously by Towers et al. (29).
