FIG. 2.
SDS-PAGE analysis and autoradiography of 35S-labeled protein extracts from Sphingomonas sp. strain CHY-1. Protein extracts were prepared as described in Materials and Methods from bacteria subjected to in vivo labeling in the presence of the following carbon sources: chrysene (lane 1), naphthalene and chrysene (lane 2), phenanthrene (lane 3), naphthalene (lane 4), and succinate (lane 5). The arrows indicate major labeled bands which were subjected to peptide analysis. For this, extracts from unlabeled cells grown on naphthalene plus chrysene or on chrysene alone were analyzed on a similar SDS-PAGE gel, and protein bands with relative mobilities identical to those of the labeled bands of interest were analyzed. P1, P2, P3, P4, and P8 are the proteins described in Table 3.